Anton Meinhart and Patrick Cramer Karim -

Patrick Cramer Anton Meinhart , Tobias Silberzahn and Phosphatase Factor Ssu 72 Is a Potential Tyrosine

Cramer Anton Meinhart , Jascha Blobel and Patrick α General Transcription Factor E / IIE An Extended Winged Helix Domain in

A structural perspective of CTD function.

The C-terminal domain (CTD) of RNA polymerase II (Pol II) integrates nuclear events by binding proteins involved in mRNA biogenesis. CTD-binding proteins recognize a specific CTD phosphorylation pattern, which changes during the transcription cycle, due to the action of CTD-modifying enzymes. Structural and functional studies of CTD-binding and -modifying proteins now reveal some of the mechani...

Structure and mechanism of RNA polymerase II CTD phosphatases.

Recycling of RNA polymerase II (Pol II) after transcription requires dephosphorylation of the polymerase C-terminal domain (CTD) by the phosphatase Fcp1. We report the X-ray structure of the small CTD phosphatase Scp1, which is homologous to the Fcp1 catalytic domain. The structure shows a core fold and an active center similar to those of phosphotransferases and phosphohydrolases that solely s...

The dynamic machinery of mRNA elongation.

Two complementary X-ray studies of the interaction between RNA polymerase II and nucleic acids have improved our understanding of mRNA elongation. These studies suggest how RNA polymerase II unwinds DNA, how it separates the RNA product from the DNA template and how it incorporates nucleoside triphosphate (NTP) substrates into the growing RNA chain. The tunable polymerase active center apparent...